DEVELOPMENT OF INDUCIBILITY FOR GLUTAMINE-SYNTHETASE IN EMBRYONIC NEURAL RETINA - INHIBITION BY BRDU

被引:52
作者
MOSCONA, M
MOSCONA, AA
机构
[1] Department of Biology and Pathology, University of Chicago, Chicago, Illinois
关键词
5-bromo-2′-deoxyuridine; BrdU; glutamine synthetase; GS; HC; hydrocortisone;
D O I
10.1111/j.1432-0436.1979.tb01579.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The hydrocortisone-mediated induction of glutamine synthetase (GS) in the neural retina of the chick embryo is a characteristic and unique feature of differentiation of this tissue. The induction involves genomic activity elicited by the inducer resulting in synthesis and accumulation of the enzyme. We describe correlations between the growth of embryonic retina tissue in vivo and in vitro and the development of its inducibility for GS, and demonstrate that this development proceeds through two phases: competence-acquisition phase(before the 7th day of development), and maturation phase. BrdU applied for 24 h to retinas of 5-day embryos irreversibly suppresses the development of induction-competence. However, BrdU does not affect the progressive maturation of inducibility when applied to retinas that already are fully induction-competent (8 days and older). The short treatment with BrdU of 5-day retinas also causes defective histogenesis resulting in drastic malformation of the tissue. The nature of the processes involved in competence-acquisition and in the maturation of inducibility for GS are examined. Possible mechanisms by which BrdU prevents the development of induction-competence for GS in the early embryonic retina and elicits defective histogenesis are discussed. © 1979, International Society of Differentiation. All rights reserved.
引用
收藏
页码:165 / 172
页数:8
相关论文
共 27 条
[1]   INHIBITION OF CARTILAGE DEVELOPMENT IN ORGAN CULTURES OF CHICK SOMITES BY THYMIDINE ANALOG, 5-BROMO-2'-DEOXYURIDINE [J].
ABBOTT, J ;
MAYNE, R ;
HOLTZER, H .
DEVELOPMENTAL BIOLOGY, 1972, 28 (02) :430-&
[2]   INDUCTION OF GLUTAMINE SYNTHETASE IN EMBRYONIC RETINA - EFFECTS OF PARTIAL AND COMPLETE INHIBITION OF RNA SYNTHESIS ON ENZYME ACCUMULATION [J].
ALESCIO, T ;
MOSCONA, M ;
MOSCONA, AA .
EXPERIMENTAL CELL RESEARCH, 1970, 61 (2-3) :342-&
[4]   RADIOIMMUNE STUDY OF EFFECT OF BROMODEOXYURIDINE ON SYNTHESIS OF PROTEOGLYCAN BY DIFFERENTIATING LIMB BUD CULTURES [J].
HO, PL ;
LEVITT, D ;
DORFMAN, A .
DEVELOPMENTAL BIOLOGY, 1977, 55 (02) :233-243
[5]  
Holtzer H, 1972, Curr Top Dev Biol, V7, P229, DOI 10.1016/S0070-2153(08)60073-3
[6]   DIFFERENTIATION IN EARLY CHICK-EMBRYO - EFFECTS OF BROMODEOXYURIDINE ON ERYTHROPOIESIS [J].
INGRAM, VM ;
CHAN, LL ;
HAGOPIAN, HK ;
LIPPKE, JA ;
WU, L .
DEVELOPMENTAL BIOLOGY, 1974, 36 (02) :411-427
[7]   CORTICOSTEROID RECEPTORS IN NEURAL RETINA AND OTHER TISSUES OF CHICK-EMBRYO [J].
KOEHLER, DE ;
MOSCONA, AA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 170 (01) :102-113
[8]   IRREVERSIBLE INHIBITION OF DIFFERENTIATION OF LIMB-BUD MESENCHYME BY BROMODEOXYURIDINE [J].
LEVITT, D ;
DORFMAN, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (05) :1253-&
[9]   MALFORMATION OF EMBRYONIC NEURAL RETINA ELICITED BY BRDU [J].
MAYERSON, PL ;
MOSCONA, AA .
DIFFERENTIATION, 1979, 13 (03) :173-184
[10]   INDUCTION OF GLUTAMINE SYNTHETASE IN CELL AGGREGATES OF EMBRYONIC NEURAL RETINA - CORRELATIONS WITH DIFFERENTATION AND MULTICELLULAR ORGANIZATION [J].
MORRIS, JE ;
MOSCONA, AA .
DEVELOPMENTAL BIOLOGY, 1971, 25 (03) :420-+