DIPHTHAMIDE SYNTHESIS IN SACCHAROMYCES-CEREVISIAE - STRUCTURE OF THE DPH2 GENE

被引：33

作者：

MATTHEAKIS, LC

SOR, F

COLLIER, RJ

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,200 LONGWOOD AVE,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,SHIPLEY INST MED,BOSTON,MA 02115

[3] INST CURIE,BIOL SECT,F-91405 ORSAY,FRANCE

来源：

GENE | 1993年 / 132卷 / 01期

关键词：

DIPHTHERIA TOXIN; ELONGATION FACTOR-II; EXOTOXIN-A; ADP-RIBOSYLATION; YEAST; FRAGMENT-A; POSTTRANSLATIONAL MODIFICATION; PROTEIN SYNTHESIS; CLONING; SEQUENCING;

D O I：

10.1016/0378-1119(93)90528-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A gene involved in diphthamide biosynthesis, DPH2, was cloned from Saccharomyces cerevisiae by complementation of a diphthamide mutant. DPH2 exists as a single-copy gene in the yeast genome and is located on the left arm of chromosome XI. Sequence analysis of the DPH2 locus predicts that the DPH2 gene product is a 534-amino acid (aa) protein, with a calculated M(r) of 59 772. This conclusion was supported by Northern blot analysis of the DPH2 transcript and gel analysis of the DPH2 protein overproduced in Escherichia coli. Gene disruption studies indicate that the DPH2 gene is not essential for viability of yeast. The role of DPH2 in diphthamide biosynthesis is discussed.

引用

页码：149 / 154

页数：6

共 22 条

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