PHOSPHOCREATINE AND ATP REGULATION IN THE HYPOXIC DEVELOPING RAT-BRAIN

被引:31
作者
TSUJI, M
ALLRED, E
JENSEN, F
HOLTZMAN, D
机构
[1] HARVARD UNIV, SCH MED, JOINT PROGRAM NEONATOL, BOSTON, MA 02115 USA
[2] CHILDRENS HOSP, DEPT NEUROL NEUROEPIDEMIOL, BOSTON, MA 02115 USA
[3] CHILDRENS HOSP, DEPT NEUROL, BOSTON, MA 02115 USA
来源
DEVELOPMENTAL BRAIN RESEARCH | 1995年 / 85卷 / 02期
关键词
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; CREATINE KINASE; ATP; BRAIN; DEVELOPMENT; HYPOXIA;
D O I
10.1016/0165-3806(94)00213-J
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Decreased brain ATP and phosphocreatine (PCr) concentrations and intracellular pH were compared in hypoxic 4-, 10-11, and 24-25-day-old rats. Surface coil P-31-nuclear magnetic resonance (NMR) spectra were acquired in vivo every minute before, during, and after 7 min of breathing 4% O-2. At all ages PCr decreased rapidly, At the two younger ages, the nucleoside triphosphate signal was still 80-85% of pre-hypoxic values, indicating 20-30% decrease in ATP, when PCr was almost fully depleted. At 24-25 days, PCr initially decreased 40-50% with an ATP loss of about 30%. Then, PCr and ATP decreased simultaneously. The decrease in brain pH was greatest at 24-25 days. More electrocortical seizure activity during hypoxia was seen at 10-11 days than at other ages. Seizure activity was seen only when ATP was less than 20% depleted and was not associated with more rapid decreases in ATP or PCr. At all ages, loss of electrocortical activity occurred when ATP was about 30% depleted. Brain creatine kinase catalyzed flux, measured by the NMR saturation transfer experiment before the hypoxic period, was 4-fold higher at 24-25 days than at 4- or 10-11 days. In conclusion, the temporally coupled depletion of PCr and ATP during hypoxia, which is characteristic of the mature brain, is seen only after the maturational increase in brain CK activity.
引用
收藏
页码:192 / 200
页数:9
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