HETEROANTISERUM AGAINST A SUB-POPULATION OF HUMAN T-LYMPHOCYTES

An anti-T cell antiserum (162) was raised to the cultured T acute lymphocytic leukemia cell line HSB2 previously precoated with antilymphocyte serum. Following absorption with cultured normal B cells antiserum 162 reacted with 30 to 70% of normal peripheral blood T lymphocytes (E-rosetting cells), and 100% of thymocytes, T chronic lymphocytic leukemia cells, and T acute lymphocytic leukemia cells. Quantitative absorption experiments indicated that 162 reacted against a common antigen(s) present on these cells. Antiserum 162 did not react with normal B lymphocytes, B cell lines, non-B non-T acute lymphocytic leukemia cells, acute myelocytic leukemia cells, B cell chronic lymphocytic leukemia cells, and T blast cells from the peripheral blood of a patient with Sezary syndrome. Two T acute lymphocytic leukemia cell lines were positive (HSB2 and 8402) and one (MOLT 4) was negative. © 1979.