ULTRASENSITIVE FLUOROMETRIC DETECTION OF CARBOHYDRATES AS DERIVATIVES IN MIXTURES SEPARATED BY CAPILLARY ELECTROPHORESIS

被引：156

作者：

LIU, JP ^{[1
]}

SHIROTA, O ^{[1
]}

WIESLER, D ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 06期

关键词：

MONOSACCHARIDES; OLIGOSACCHARIDES; 3-(4-CARBOXYBENZOYL)-2-QUINOLINECARBOXALDEHYDE; CAPILLARY ZONE ELECTROPHORESIS; LASER DETECTION;

D O I：

10.1073/pnas.88.6.2302

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide solutes were assessed at low attomole levels (0.5 amol for the CBQCA derivative of galactose). The system has shown considerable promise for high-sensitivity analysis of both neutral and amino sugars in glycoproteins. Complex oligosaccharides, isolated from bovine fetuin by hydrazinolysis, were also successfully "mapped."

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页码：2302 / 2306

页数：5

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