DETECTION OF CLOSTRIDIUM-PERFRINGENS TYPE-A ENTEROTOXIN IN FECAL AND FOOD SAMPLES USING IMMUNOMAGNETIC SEPARATION (IMS)-ELISA

被引：35

作者：

CUDJOE, KS ^{[1
]}

THORSEN, LI ^{[1
]}

SORENSEN, T ^{[1
]}

RESELAND, J ^{[1
]}

OLSVIK, O ^{[1
]}

GRANUM, PE ^{[1
]}

机构：

[1] NORWEGIAN COLL VET MED,OSLO 1,NORWAY

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1991年 / 12卷 / 04期

关键词：

DETECTION; CLOSTRIDIUM-PERFRINGENS TYPE-A ENTEROTOXIN; IMMUNOMAGNETIC SEPARATION (IMS)-ELISA;

D O I：

10.1016/0168-1605(91)90145-F

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.

引用

页码：313 / 322

页数：10

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