ANALYSIS OF PHENPROCOUMON AND ITS HYDROXYLATED AND CONJUGATED METABOLITES IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION

被引：16

作者：

EDELBROEK, PM

VANKEMPEN, GMJ

HESSING, TJ

DEWOLFF, FA

机构：

[1] STATE UNIV LEIDEN HOSP,TOXICOL LAB,2333 AA LEIDEN,NETHERLANDS

[2] PSYCHIAT HOSP ENDEGEEST,BIOCHEM LAB,OEGSTGEEST,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)82337-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The anticoagulant phenprocoumon is mainly metabolized in humans to hydroxylated metabolites and their glucuronides. A method is described for the determination of phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon, 7-hydroxyphenprocoumon, and their glucuronide and sulphate conjugates in human urine. Reversed-phase high-performance liquid chromatography is performed after selective extraction with disposable quaternary amine columns of untreated, and β-glucuronidase- or sulphatase-treated urine samples. Urinary excretion data are presented for total, glucuronidated, sulphated and free phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon and 7-hydroxyphenprocoumon in twelve patients after an average daily dosage of 1.3-4.2 mg phenprocoumon. © 1990.

引用

页码：347 / 358

页数：12

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