CORALLINE SHAPE OF THE BACTERIAL NUCLEOID AFTER CRYOFIXATION

被引：46

作者：

BOHRMANN, B ^{[1
]}

VILLIGER, W ^{[1
]}

JOHANSEN, R ^{[1
]}

KELLENBERGER, E ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT MICROBIOL,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 10期

关键词：

D O I：

10.1128/JB.173.10.3149-3158.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.

引用

页码：3149 / 3158

页数：10

共 43 条

[1] GRAPHIC RECONSTRUCTION OF THE 3RD DIMENSION FROM SERIAL ELECTRON MICROPHOTOGRAPHS
BANG, BG
BANG, FB
[J]. JOURNAL OF ULTRASTRUCTURE RESEARCH, 1957, 1 (02): : 138 - 146
[2] BASCHONG W, 1984, EUR J CELL BIOL, V35, P21
[3] BJORNSTI MA, 1986, BACTERIAL CHROMATIN, P64
[4] ETUDE DE LACTION DES MEMBRANES DU BACTERIOPHAGES-T2 SUR ESCHERICHIA-COLI
BONIFAS, V
KELLENBERGER, E
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1955, 16 (03) : 330 - 338
[5] INTERACTION OF THE ESCHERICHIA-COLI HU PROTEIN WITH DNA - EVIDENCE FOR FORMATION OF NUCLEOSOME-LIKE STRUCTURES WITH ALTERED DNA HELICAL PITCH
BROYLES, SS
PETTIJOHN, DE
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1986, 187 (01) : 47 - 60
[6] DRLICA K, 1987, ESCHERICHIA COLI SAL, V1, P91
[7] ELECTRON-MICROSCOPY OF FROZEN-HYDRATED BACTERIA
DUBOCHET, J
MCDOWALL, AW
MENGE, B
SCHMID, EN
LICKFELD, KG
[J]. JOURNAL OF BACTERIOLOGY, 1983, 155 (01) : 381 - 390
[8] DURRENBERGER M, 1988, J BACTERIOL, V170, P4757
[9] REMOVAL OF BACKGROUND LABEL IN IMMUNOCYTOCHEMISTRY WITH THE APOLAR LOWICRYLS BY USING WASHED PROTEIN A-GOLD-PRECOUPLED ANTIBODIES IN A ONE-STEP PROCEDURE
DURRENBERGER, MB
[J]. JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1989, 11 (02): : 109 - 116
[10] CLEAVAGE AND CROSS-LINKING OF PROTEINS WITH OSMIUM (VIII) REAGENTS
EMERMAN, M
BEHRMAN, EJ
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (04) : 395 - 397

← 1 2 3 4 5 →