NPI-1,THE HUMAN HOMOLOG OF SRP-1, INTERACTS WITH INFLUENZA-VIRUS NUCLEOPROTEIN

被引：139

作者：

ONEILL, RE ^{[1
]}

PALESE, P ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT MICROBIOL,NEW YORK,NY 10029

来源：

VIROLOGY | 1995年 / 206卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0042-6822(95)80026-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We used the yeast interactive trap system to identify a cellular protein which interacts with the nucleoprotein of influenza A viruses. This protein, nucleoprotein interactor 1 (NPI-1) is the human homolog of the yeast protein SRP1. SRP1 was previously identified as a suppressor of temperature-sensitive RNA polymerase I mutations (R. Yano, M. Oakes, M. Yamaghishi, J. Dodd, and M. Nomura, Mol. Cell. Biol. 12, 5640-5651, 1992). A full-length cDNA clone of NPI-1 was generated from HeLa cell poly A+ RNA. The viral nucleoprotein, which had been partially purified from influenza A/PR/8/34 virus-infected embryonated eggs, could be coprecipitated from solution by glutathione agarose beads complexed with a bacterially expressed glutathione-S-transferase-NPI-1 fusion protein, confirming the results of the yeast genetic system. Antisera raised against NPI-1 identified a 80-kDa polypeptide from total cellular extracts of both HeLa and MDBK cells. The viral nucleoprotein was coimmunoprecipitated from influenza A/WSN/33 virus-infected MDBK cells by anti-NPI-1 sera, demonstrating an interaction of these two proteins in infected cells. Similarly, NPI-1 was coimmunoprecipitated from MDBK cells by anti-NP sera. These experiments suggest that NPI-1 plays a role during influenza virus replication. (C) 1885 Academic Press, Inc

引用

页码：116 / 125

页数：10

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