CHEMICAL COUPLING OF YEAST HEXOKINASE TO SEPHADEX

被引：8

作者：

BOHNENSACK, R

AUGUSTIN, W

HOFMANN, E

机构：

[1] Physiologisch-chemisches Institut der Medizinischen Akademie Magdeburg, Germany (Democratic Republic

[2] Physiologisch-chemisches Institut der Karl-Marx-Universität, 701 Leipzig, Germany (Democratic Republic

来源：

EXPERIENTIA | 1969年 / 25卷 / 04期

关键词：

D O I：

10.1007/BF01899906

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Yeast hexokinase was coupled to Sephadex by means of cyanogen bromide. The activity of the fixed enzyme was 2% of that before coupling. The activity of the noncoupled part of the enzyme was also decreased to the same degree. This decrease cannot be caused by the coupling reaction itself. The Michaelis-constants and the pH-activity curve of the coupled enzyme do not differ significantly from that of the free enzyme. © 1969 Birkhäuser-Verlag.

引用

页码：348 / +

页数：1

共 10 条

[1] CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES
AXEN, R
PORATH, J
ERNBACK, S
[J]. NATURE, 1967, 214 (5095) : 1302 - &
[2] BOHNENSACK R, IN PRESS
[3] SARCOPLASMIC RETICULUM ATPASE ON A SOLID SUPPORT
BROWN, HD
CHATTOPADHYAY, SK
PATEL, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 25 (03) : 304 - +
[4] CROOK EM, 1968, BIOCHEM J, V107, pP1
[5] SOME CHANGES IN REACTIVITY OF ENZYMES RESULTING FROM THEIR CHEMICAL ATTACHMENT TO WATER-INSOLUBLE DERIVATIVES OF CELLULOSE
HORNBY, WE
LILLY, MD
CROOK, EM
[J]. BIOCHEMICAL JOURNAL, 1968, 107 (05) : 669 - +
[6] LILLY MD, 1968, BIOCHEM J, V107, pP5
[7] MANECKE G, 1968, BIOCHEM J, V107, pP2
[8] WATER-INSOLUBLE DERIVATIVES OF ENZYMES ANTIGENS AND ANTIBODIES
SILMAN, IH
KATCHALSKI, E
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1966, 35 : 873 - +
[9] WHITTAM R, 1968, BIOCHEM J, V107, pP3
[10] PREPARATION AND KINETICS OF LACTATE DEHYDROGENASE ATTACHED TO WATER-INSOLUBLE PARTICLES AND SHEETS
WILSON, RJH
KAY, G
LILLY, MD
[J]. BIOCHEMICAL JOURNAL, 1968, 108 (05) : 845 - +

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