FERREDOXIN DEGRADATION IN GROWING CLOSTRIDIUM-PASTEURIANUM DURING PERIODS OF IRON DEPRIVATION

被引:35
作者
SCHONHEIT, P
BRANDIS, A
THAUER, RK
机构
[1] Fachbereich Biologie-Mikrobiologie, Philipps-Universität, Marburg
关键词
Clostridium pasteurianum; Ferredoxin; Iron metabolism; Iron-sulfur proteins; Pyruvate synthase;
D O I
10.1007/BF00413277
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Clostridium pasteurianum was grown in batch cultures on media with an initial iron concentration of 10 μM. The uptake of iron and the synthesis of ferredoxin was followed. All the iron present in the medium was taken up by the cells before 50% of the final cell density was attained. The bacteria then continued to grow in the complete absence of exogenous iron. Ferredoxin was synthesized during growth until the exogenous iron concentration dropped below 1 μM. During growth in the absence of iron ferredoxin was degraded with the result that at the end of growth the cells did not contain ferredoxin. The specific activity of the iron sulfur protein, pyruvate synthase (E.C. 1.2.7.1), remained constant during growth of C. pasteurianum in the absence of exogenous iron. This finding suggests that ferredoxin was used as an endogenous source of iron for the synthesis of essential iron proteins during periods of iron deprivation. © 1979 Springer-Verlag.
引用
收藏
页码:73 / 76
页数:4
相关论文
共 16 条
[1]  
BRODRICK JW, 1977, IRON SULFUR PROTEINS, V3, P101
[2]   PURIFICATION AND PROPERTIES OF HYDROGENASE FROM CLOSTRIDIUM-PASTEURIANUM W5 [J].
CHEN, JS ;
MORTENSO.LE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 371 (02) :283-298
[3]   SIMPLIFIED METHODS FOR ESTIMATION OF IRON IN MITOCHONDRIA AND SUBMITOCHONDRIAL FRACTIONS [J].
DOEG, KA ;
ZIEGLER, DM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1962, 97 (01) :37-&
[4]   INTRACELLULAR PROTEIN DEGRADATION IN MAMMALIAN AND BACTERIAL-CELLS .2. [J].
GOLDBERG, AL ;
STJOHN, AC .
ANNUAL REVIEW OF BIOCHEMISTRY, 1976, 45 :747-803
[5]  
HONG JS, 1970, J BIOL CHEM, V245, P4982
[6]  
KNIGHT E, 1966, J BIOL CHEM, V241, P2752
[7]  
KNIGHT E, 1967, J BIOL CHEM, V242, P1370
[8]  
LIGHT PA, 1974, MICROBIAL IRON METAB, P35
[9]   RUBREDOXIN - A NEW ELECTRON TRANSFER PROTEIN FROM CLOSTRIDIUM PASTEURIANUM [J].
LOVENBERG, W ;
SOBEL, BE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (01) :193-+
[10]  
MORTENSON LE, 1973, IRON SULPHUR PROTEIN, V1, P37