PROSTAGLANDIN AND THROMBOXANE PRODUCTION BY HUMAN AND GUINEA-PIG MACROPHAGES AND LEUKOCYTES

被引：114

作者：

MORLEY, J ^{[1
]}

BRAY, MA ^{[1
]}

JONES, RW ^{[1
]}

NUGTEREN, DH ^{[1
]}

VANDORP, DA ^{[1
]}

机构：

[1] UNILEVER RES LAB,VLAARDINGEN,NETHERLANDS

来源：

PROSTAGLANDINS | 1979年 / 17卷 / 05期

关键词：

ELECTRON-CAPTURE DETECTION; LEUCOCYTES; MACROPHAGES; MASS SPECTROMETRY; PROSTAGLANDIN; -; BIOSYNTHESIS; THROMBOXANE;

D O I：

10.1016/S0090-6980(79)80044-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of partially purified human and guinea-pig haematogenous cell populations, when cultured in vitro, to metabolise arachidonic acid (AA) has been studied. Supernatants from 24 hour cell culture have been subjected to analysis for products of AA metabolism by gas chromatography with electron-capture detection. The cell types studied were human peripheral blood monocytes (both glass adherent and non-adherent), neutrophils, eosinophils and leukemic leucocytes; thoracic duct lymphocytes and lung alveolar macrophages. From the guinea-pig, induced and non-induced macrophage or neutrophil enriched peritoneal exudate populations, lymph node cells, peritoneal eosinophils and peripheral blood platelets were examined. Supernatants were assayed for the presence of PGE2, PGD2, PGF2α, TXB2 and 6-keto-PGF1α. In all types studied PGE2 and TXB2 were the major products formed. The identification of PGE2 and TXB2 was confirmed by GC/MS with multiple ion monitoring. The results have been compared with other reports and their possible significance discussed in relation to the proposed role of prostaglandins as mediators and modulators in immunopathology. © 1979.

引用

页码：729 / 736

页数：8

共 28 条

[1]

BOOT JR, 1977, BRIT J PHARMACOL, V59, pP444

[2]

BRAY M, UNPUBLISHED

[3]

BRAY MA, 1974, BRIT J PHARMACOL, V52, pP453

[4] PROSTAGLANDIN PRODUCTION BY MACROPHAGES AND EFFECT OF ANTI-INFLAMMATORY DRUGS [J].