SENESCENT HUMAN-DIPLOID CELLS IN CULTURE - SURVIVAL, DNA-SYNTHESIS AND MORPHOLOGY

被引：120

作者：

MATSUMURA, T

ZERRUDO, Z

HAYFLICK, L

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT MED,DIV INFECT DIS,STANFORD,CA 94305

[2] CHILDRENS HOSP MED CTR NO CALIF,BRUCE LYON MEM RES LAB,OAKLAND,CA 94609

来源：

JOURNALS OF GERONTOLOGY | 1979年 / 34卷 / 03期

关键词：

D O I：

10.1093/geronj/34.3.328

中图分类号：

R4 [临床医学]; R592 [老年病学];

学科分类号：

1002 ; 100203 ; 100602 ;

摘要：

Senescent human diploid cell cultures (WI-38 and WI-26) were studied during Phase III for survival time, ability to synthesize DNA, and nuclear morphology. Periodic transfers made during a 6-month period of Phase III showed that cultures were maintained with only slight variations in cell number. No sign of multinucleated cells found during the period of observation showed that progressive changes occur in Phase III. A certain proportion of cells maintained the ability to incorporate tritiated thymidine throughout the period of observation.

引用

页码：328 / 334

页数：7

共 23 条

[1] GENEALOGIES OF CLONES OF DIPLOID FIBROBLASTS - CINEMICROPHOTOGRAPHIC OBSERVATIONS OF CELL-DIVISION PATTERNS IN RELATION TO POPULATION AGE [J].