STUDY OF THE CHARACTERISTICS AND ANALYTICAL APPLICATION OF A CAPILLARY CHROMATOGRAPH WITH AN ELECTROCHEMICAL DETECTOR

The characteristics of a capillary liquid chromatograph consisting of a syringe pump, microinjector, fused-silica capillary columns of I.D. 0.2-0.4 mm packed with different sorbents and an electrochemical detector of the amperometric type were investigated. The relative standard deviation of the retention times was <0.3% and that of the height of the chromatographic peak was <1%. The detection limit for catecholamines was 0.1 pg. Low extra-column spreading makes it possible to attain a separation efficiency with a minimum reduced plate height of 2.1-2.9. Examples are given of the application of the capillary liquid chromatograph to the separation of phenol compounds and amino acids in model and real samples. © 1990.