INHIBITION OF D-3-AMINOISOBUTYRATE PYRUVATE AMINOTRANSFERASE BY 5-FLUOROURACIL AND ALPHA-FLUORO-BETA-ALANINE

被引：8

作者：

KANEKO, M ^{[1
]}

KONTANI, Y ^{[1
]}

KIKUGAWA, M ^{[1
]}

TAMAKI, N ^{[1
]}

机构：

[1] KOBE GAKUIN UNIV,FAC NUTR,NISHI KU,KOBE 65121,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1122卷 / 01期

关键词：

D-3-AMINOISOBUTYRATE PYRUVATE AMINOTRANSFERASE; 5-FLUOROURACIL; ALPHA-FLUORO-BETA-ALANINE; D-3-AMINOISOBUTYRATE; BETA-ALANINE; 4-AMINOBUTYRATE AMINOTRANSFERASE; (RAT);

D O I：

10.1016/0167-4838(92)90125-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among pyrimidine derivatives, we found that 5-fluorouracil potently inhibited purified rat liver D-3-aminoisobutyrate-pyruvate aminotransferase, whereas 5-fluorouridine did so to a much lesser extent. 5-Fluorouracil acted as a competitive inhibitor against beta-alanine with a K(i) of 56-mu-M, and was uncompetitive against pyruvic acid, with a K(i) of 73-mu-M. Alpha-Fluoro-beta-alanine, a metabolite of 5-fluorouracil, was also a competitive inhibitor with respect to beta-alanine with a K(i) of 8.0 mM. 5-Fluorouracil acted also as a competitive inhibitor of 4-aminobutyrate aminotransferase with respect to beta-alanine with a K(i) value of 1.9 mM, and was uncompetitive against 2-oxoglutaric acid, with a K(i) of 1.8 mM.

引用

页码：45 / 49

页数：5

共 23 条

[1]

FINK K, 1956, J BIOL CHEM, V221, P425

[2]

FINK RM, 1956, J BIOL CHEM, V218, P1

[3] THE LEVEL OF BETA-ALANINE AMINOTRANSFERASE ACTIVITY IN REGENERATING AND DIFFERENTIATING RAT-LIVER [J].