THE PREDICTIVE FERTILIZATION VALUE OF THE HYPOOSMOTIC SWELLING TEST (HOST) FOR FRESH AND CRYOPRESERVED SPERM

被引：15

作者：

HAUSER, R ^{[1
]}

YAVETZ, H ^{[1
]}

PAZ, GF ^{[1
]}

HOMONNAI, ZT ^{[1
]}

AMIT, A ^{[1
]}

LESSING, JB ^{[1
]}

PEYSER, MR ^{[1
]}

YOGEV, L ^{[1
]}

机构：

[1] TEL AVIV UNIV,SERLIN MATERN HOSP,SACKLER FAC MED,TEL AVIV SOURASKY MED CTR,INST STUDY FERTIL,TEL AVIV 67441,ISRAEL

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 03期

关键词：

INVITRO FERTILIZATION-EMBRYO TRANSFER; SPERM CHARACTERISTICS; CRYOPRESERVATION; INFERTILITY;

D O I：

10.1007/BF01203826

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background. The hypoosmotic swelling test (HOST) was recommended as a predictive test for in vitro fertilization (IVF) outcome. These results, however, were controversial and the results for thawed semen were insufficient. The present study was conducted in order to clarify the predictive value of the HOST for IVF in fresh and thawed sperm. Methods: The hypoosmotic swelling test was performed in three groups: husband's fresh semen "subfertile" group, donor's thawed semen group, and donor's fresh semen "fertile" group. Results: No correlation was found between HOST values and sperm characteristics in fresh or thawed sperm. Fresh sperm HOST values correlated with IVF fertilizations. No such correlations were found when thawed sperm was used. HOST values were significantly higher in the fresh fertile donor group than in the fresh subfertile group (P < 0.001). Following the freezing and thawing process, HOST values decreased dramatically. Nevertheless, the fertilization rate was still higher compared to that of the fresh subfertile group (P < 0.001). There were significantly more IVF cycles with no fertilizations when HOST values were below 45%. Conclusion: The HOST has a predictive value for fertilization of oocytes in IVF cycles when fresh semen, but not thawed sperm, is used. The freezing-thawing process affects the outer membrane of the spermatozoon and changes its characteristics, leading to a decrease in HOST values. Sperm characteristics that play a role in the fertilization process are not expressed directly by HOST values.

引用

页码：265 / 270

页数：6

共 16 条

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