CIRCADIAN-RHYTHM OF THE ENDOPEPTIDASE 22.19 (EC 3.4.22.19) IN THE RAT-BRAIN

被引：9

作者：

FERRO, ES

SUCUPIRA, M

MARQUES, N

CAMARGO, ACM

MENNABARRETO, L

机构：

[1] Departamento de Biofisica, Escola Paulista de Medicina, Universidade de São Paulo, Sao Paulo

[2] Departamento de Farmacologia, Universidade de São Paulo, Sao Paulo

[3] Grupo Multidisciplinar de Desenvolvimento e Ritmos Biológicos (GMDRB), Instituto de Ciěncias Biomédicas, Sao Paulo

[4] Depariamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, Sao Paulo

来源：

CHRONOBIOLOGY INTERNATIONAL | 1992年 / 9卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

CIRCADIAN RHYTHM; ENDOPEPTIDASE; 22.19; EC; 3.4.22.19; PEPTIDE METABOLISM; OPIOID PEPTIDES; ENKEPHALINS-RAT BRAIN;

D O I：

10.3109/07420529209064533

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The endopeptidase 22.19 (EC 3.4.22.19) has been associated with the metabolism of neuropeptides by its ability to convert small enkephalin-containing peptides (8 to 13 amino acids) into enkephalins. In addition, this enzyme cleaves the Arg8-Arg9 bond of neurotensin and the Phe5-Ser6 bond of bradykinin. We analyzed the circadian variation of endopeptidase 22.19 in the whole and individual areas of the rat brain. Endopeptidase 22.19 activity was analyzed by high-performance liquid chromatography (HPLC) using bradykinin as an operative substrate. Enzymatic specific activities were analyzed by rhythmometric methods and indicate a circadian fluctuation of endopeptidase 22.19 specific activity (mU of enzyme/mg of protein) in the whole brain [p < 0.001, mesor (M) = 7.62, amplitude (A) = 2.89, and acrophase (phi) = 23:08 h], striatum (p < 0.001, M = 2.92, A = 0.62, phi-23:03 h), hypothalamus (p < 0.001, M = 3.15, A = 0.86, phi-01:12 h), periaqueductal gray matter (p < 0.005, M = 2.62, A = 0.34, phi = 22:35 h), and cerebellum (p < 0.0 14, M = 4.27, A = 0.88, phi = 17:12 h). The circadian rhythmicity in endopeptidase 22.19 specific activity suggests that light may have an effect on the peptidase activity in whole brain and in areas of the central nervous system and may be essential for the mechanisms of circadian fluctuations of neuropeptides in the brain.

引用

页码：243 / 249

页数：7

共 28 条

[1]

Camargo A.C.M., Shapanka R., Greene L.J., Preparation, assay and partial characterization of a neutral endopeptidase from rabbit brain, Biochemistry, 12, pp. 1838-1844, (1973)

[2]

Camargo A.C.M., Oliveira E.B., Toffoletto O., Metters K.M., Rossier J., Brain endo-oligopeptidase A, a putative enkephalin converting enzyme, J. Neurochem, 48, pp. 1258-1263, (1987)

[3]

Carvalho K.M., Camargo A.C.M., Purification of rabbit brain endooligopeptidases and preparation of anti-enzyme antibodies, Biochemistry, 20, pp. 7082-7088, (1981)

[4]

Camargo A.C.M., Almeida M.I.X., Emson P.C., Involvement of endo-oligopeptidase A and B in the degradation of neurotensin by rabbit brain, J Neurochem, 42, pp. 1758-1761, (1984)

[5]

Oliveira S.E., Leite P.E.P., Spillantini M.G., Camargo A.C.M., Hunt P.S., Localization of endo-oligopeptidase (EC 3.4.22.19.) in the rat nervous tissue, J Neurochem, 55, pp. 1114-1121, (1990)

[6]

Ferro E.S., Hamassaki D.E., Camargo A.C.M., Britto L.R.G., Endooligopeptidase A, a putative enkephalin generator enzyme, in the vertebrate retina, J Neurochem, 57, pp. 1643-1649, (1991)

[7]

Albers H.E., Liou S., Stopa E.G., Zoeller R.T., Interaction of colocalized neuropeptides: functional significance in the circadian timing system, J Newoscience, 11, pp. 846-851, (1991)

[8]

Kurumaji A., Takashima M., Ohi K., Takahashi K., Circadian fluctuations in pain responsiveness and brain met-enkephalin-like immunoreactivity in the rat, Pharmacol Biochem Behav, 29, pp. 595-599, (1988)

[9]

Rusak B., Bina K.G., Neurotransmitters in the mammalian circadian system, Annu Rev Neurosci, 13, pp. 387-401, (1990)

[10]

Frederickson R.C.A., Burgis V., Edwards J.D., Hyperalgesia induced by naloxone follows diurnal rhythm in responsivity to painful stimuli, Science, 198, pp. 756-758, (1977)

← 1 2 3 →