ABERRANT GENE FOR EL-TOR HEMOLYSIN FROM VIBRIO-CHOLERAE NON-O1, N037

被引：5

作者：

HONMA, Y

YAMAMOTO, K

IWANAGA, M

机构：

[1] OSAKA UNIV, MICROBIAL DIS RES INST, DEPT BACTERIAL INFECT, SUITA, OSAKA 565, JAPAN

[2] UNIV RYUKYUS, FAC MED, COMPREHENS MED RES INST, NISHIHARA, OKINAWA 90301, JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 1-2期

关键词：

VIBRIO CHOLERAE NON-01; HEMOLYSIN; INSERTIONAL MUTATION;

D O I：

10.1016/0378-1097(95)00354-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vibria cholerae non-Ol strain N037 produced a hemolysin (N037-Hly) which was antigenically similar to El Tor hemolysin (El Tor-Hly) but different in molecular size; hemolytic activity, and glucose binding capacity. In the gene encoding N037-Hly, a 4-bp insertion into the structural gene for El Tor-Hly (hlyA) was found. The insertion resulted in a shift of codon frames generating a new stop codon in the downstream region. N037-Hly was a truncated product of El Tor-Hly sharing 90% of the N terminal region. This suggested that the 10% C-terminal region of El Tor-Hly is needed for the maximal hemolytic activity and glucose binding capacity.

引用

页码：151 / 154

页数：4

共 12 条

[1] CHARACTERIZATION OF THE HLYB GENE AND ITS ROLE IN THE PRODUCTION OF THE EL-TOR HEMOLYSIN OF VIBRIO-CHOLERAE O1 [J].