HUMAN DELTA-OPIOID RECEPTOR - A STABLE CELL-LINE FOR FUNCTIONAL-STUDIES OF OPIOIDS

被引：28

作者：

MALATYNSKA, E

WANG, Y

KNAPP, RJ

SANTORO, G

LI, XP

WAITE, S

ROESKE, WR

YAMAMURA, HI

机构：

[1] UNIV ARIZONA,DEPT BIOCHEM,TUCSON,AZ 85724

[2] UNIV ARIZONA,DEPT MED,TUCSON,AZ 85724

[3] UNIV ARIZONA,DEPT PSYCHIAT,TUCSON,AZ 85724

[4] UNIV ARIZONA,ARIZONA HLTH SCI CTR,TUCSON,AZ 85724

来源：

NEUROREPORT | 1995年 / 6卷 / 04期

关键词：

STABLE EXPRESSION; TRANSFECTION; CHO CELLS; HUMAN DELTA OPIOID RECEPTOR; NALTRINDOLE; DPDPE; RADIOLIGAND BINDING; CAMP; ADENYLYL CYCLASE;

D O I：

10.1097/00001756-199503000-00007

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A HUMAN delta opioid receptor cDNA clone (pREP10/hDOR) was transfected into Chinese hamster ovary (CHO) cells. The stable cell line expressed a high density of delta opioid receptors (137 000 +/- 21 600 receptors/cell). DPDPE inhibited 90% of the forskolin-stimulated cAMP accumulation in these cells with high potency (EC(50) = 1.3 nM). This effect of DPDPE was antagonized by naltrindole. The pseudo-pA(2) value (K-e) of 155 pM for naltrindole is consistent with that measured for delta receptor antagonism in the mouse vas deferens. This is the first detailed characterization of DPDPE activity on forskolin-stimulated cAMP accumulation mediated through a human delta opioid receptor. The data support the use of the recombinant cell line for functional studies of opioid drugs.

引用

页码：613 / 616

页数：4

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