POLYNUCLEOTIDES .57. SYNTHESIS AND PROPERTIES OF POLY (2'-CHLORO-2'-DEOXYINOSINIC ACID)

Poly(2′-chloro-2′-deoxyinosinic acid) [poly (Icl)] was synthesized from Icl 5′ -DP by polymerization with polynudleotide phosphorylase. UV absorption properties of poly (Icl) are very similar to those of poly (I). Poly (Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36°, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) · poly (C). Thus it was concluded that the chlorine substitution at 2′-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides. © 1979 Information Retrieval Limited.