STRUCTURE OF THE GENE ENCODING BETA-1,3-GLUCANASE-A1 OF BACILLUS-CIRCULANS WL-12

被引：71

作者：

YAHATA, N

WATANABE, T

NAKAMURA, Y

YAMAMOTO, Y

KAMIMIYA, S

TANAKA, H

机构：

[1] NIIGATA UNIV,GRAD SCH SCI & TECHNOL,DEPT BIOSYST SCI,8050 IKARASHI 2,NIIGATA 95021,JAPAN

[2] NIIGATA UNIV,FAC AGR,DEPT AGR CHEM,NIIGATA 95021,JAPAN

来源：

GENE | 1990年 / 86卷 / 01期

关键词：

amino acid sequence homology; Nucleotide sequence; promoter; recombinant DNA; signal sequence; start codon;

D O I：

10.1016/0378-1119(90)90122-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequence of the glcA gene encoding the precursor of extracellular β-1,3-glucanase (βGl), Al, a polysaccharidase produced by Bacillus circulans WL-12, was determined. The putative glcA gene was 2046 bp long, encoding a polypeptide of 682 amino acids (aa). The N-terminal aa sequence of βGl produced in Escherichia coli harboring the glcA plasmid was identical to that of βGl A1 prepared from the culture fluid of BitB. circulans WL-12. In both proteins, cleavage of the signal sequence of pre-βGl occurred between Ala-38 and Ala-39 of the predicted sequences. © 1990.

引用

页码：113 / 117

页数：5

共 9 条

[1] THE BETA-GLUCANASE GENE FROM BACILLUS-AMYLOLIQUEFACIENS SHOWS EXTENSIVE HOMOLOGY WITH THAT OF BACILLUS-SUBTILIS [J].