SEPARATION OF PRE-COLUMN ORTHO-PHTHALALDEHYDE DERIVATIZED AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：59

作者：

HODGIN, JC

机构：

[1] Micromeritics Instrument Corporation, Norcross, 30093

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 07期

关键词：

D O I：

10.1080/01483917908060123

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented in which a pre-column fluorescent derivative of primary amino acids is formed using o-phthalaldehyde (OPA). After a one minute derivatization period, the OPA amino acids are separated by high performance liquid chromatography using a ternary gradient in the reversed phase mode and fluorometric detection. The complete analysis time, including the derivatization step is less than 40 minutes with an average minimum detectable quantity of 40 picograms. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：1047 / 1059

页数：13

共 12 条

[1] ORTHO-PHTHALALDEHYDE - FLUOROGENIC DETECTION OF PRIMARY AMINES IN PICOMOLE RANGE - COMPARISON WITH FLUORESCAMINE AND NINHYDRIN [J].