AMINO-ACID-SEQUENCE OF THE ACIDIC KUNITZ-TYPE TRYPSIN-INHIBITOR FROM WINGED BEAN SEED [PSOPHOCARPUS-TETRAGONOLOBUS (L) DC]

被引：11

作者：

CALDWELL, JB ^{[1
]}

STRIKE, PM ^{[1
]}

KORTT, AA ^{[1
]}

机构：

[1] CSIRO,DIV BIOTECHNOL,PARKVILLE LAB,343 ROYAL PARADE,PARKVILLE,VIC 3052,AUSTRALIA

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1990年 / 9卷 / 04期

关键词：

amino acid sequence; Kunitz-type inhibitor; Psophocarpus tetragonolobus (L.) DC; Trypsin inhibitor; winged bean;

D O I：

10.1007/BF01024626

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary sequence of trypsin inhibitor-2 (WBTI-2) from Psophocarpus tetragonolobus (L.) DC seeds was determined. This inhibitor consists of a single polypeptide chain of 182 amino acids, including four half-cystine residues, and an N-terminal residue of pyroglutamic acid. The sequence of WBTI-2 showed 57% identity to the basic trypsin inhibitor (WBTI-3) and 50% identity to the chymotrypsin inhibitor (WBCI) of winged bean, and 54% identity to the trypsin inhibitor DE-3 from Erythrina latissima seed. The similarity to the soybean Kunitz trypsin inhibitor (40%) and the other Kunitz-type inhibitors from Adenanthera pavonina (30%) and wheat (26%) was much lower. Sequence comparisons indicate that the Psophocarpus and Erythrina inhibitors are more closely related to each other than to other members of the Kunitz inhibitor family. © 1990 Plenum Publishing Corporation.

引用

页码：493 / 499

页数：7

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