EVIDENCE FOR MULTIPLE GENES-CODING FOR THE ISOZYMES OF HEXOKINASE IN THE HIGHLY GLYCOLYTIC AS-30D RAT HEPATOMA

被引：7

作者：

WIGLEY, WC ^{[1
]}

NAKASHIMA, RA ^{[1
]}

机构：

[1] TEXAS TECH UNIV,DEPT CHEM & BIOCHEM,POB 4260,LUBBOCK,TX 79409

来源：

FEBS LETTERS | 1992年 / 300卷 / 02期

关键词：

HEXOKINASE; ISOZYME; POLYMORPHISM; AS-30D HEPATOMA;

D O I：

10.1016/0014-5793(92)80185-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have compared Southern blots of rat hepatoma DNA probed with Types I, II and III hexokinase cDNAs isolated from normal rat tissues. Hybridization patterns show several fragments recognized by both the Type I and II clones while no resemblance is observed between the Type III probe and the other two isozymes. It therefore appears that Type I-like and Type II-like hepatoma isozymes are coded for by similar yet separate genes, while a dissimilar third gene codes for the Type III-like isozyme. In addition, a loss of heterozygosity was detected at the Type III locus in the AS-30D hepatoma when compared to normal tissue. As only the Type II-like isozyme is highly expressed in highly glycolytic tumors, these data have implications for differential gene regulation between the tumor isozymes.

引用

页码：153 / 156

页数：4

共 16 条

[1]

BUSTAMANTE E, 1981, J BIOL CHEM, V256, P8699

[2] A RECOVERED AVIAN MYELOCYTOMATOSIS VIRUS THAT INDUCES LYMPHOMAS IN CHICKENS - PATHOGENIC PROPERTIES AND THEIR MOLECULAR-BASIS [J].

ENRIETTO, PJ ;

PAYNE, LN ;

HAYMAN, MJ .

CELL, 1983, 35 (02) :369-379

[3] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[4]

GRANNER D, 1990, J BIOL CHEM, V265, P10173

[5] RAT GLUCOKINASE GENE - STRUCTURE AND REGULATION BY INSULIN [J].

MAGNUSON, MA ;

ANDREONE, TL ;

PRINTZ, RL ;

KOCH, S ;

GRANNER, DK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :4838-4842

[6]

NAKASHIMA RA, 1984, CANCER RES, V44, P5702

[7]

NAKASHIMA RA, 1988, CANCER RES, V48, P913

[8]

PARRY DM, 1983, J BIOL CHEM, V258, P904

[9]

Sambrook J., 1989, MOL CLONING

[10] COMPLETE AMINO-ACID SEQUENCE OF RAT-BRAIN HEXOKINASE, DEDUCED FROM THE CLONED CDNA, AND PROPOSED STRUCTURE OF A MAMMALIAN HEXOKINASE [J].

SCHWAB, DA ;

WILSON, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2563-2567

← 1 2 →