RAPID MAPPING OF COSMID CLONES ON PIG CHROMOSOMES BY FLUORESCENCE IN-SITU HYBRIDIZATION

被引：54

作者：

YERLE, M

GOUREAU, A

GELLIN, J

LETISSIER, P

MORAN, C

机构：

[1] UNIV SYDNEY,DEPT ANIM SCI,SYDNEY,NSW 2006,AUSTRALIA

[2] ECOLE NATL FORMAT AGRON,F-31326 CASTANET TOLOSAN,FRANCE

来源：

MAMMALIAN GENOME | 1994年 / 5卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1007/BF00360565

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nineteen cosmids have been mapped to pig chromosomes by fluorescence in situ hybridization. Two kinds of cosmid clones were isolated as potential physical and genetic markers for the pig genome. Anonymous cosmids were obtained by screening a commercial cosmid library and were localized to Chromosomes (Chrs) 1, 2, 6, 7, 8, 10, 11, 12, 13, and 14. Some of these cosmids were found to reveal RFLP type DNA polymorphism. Microsatellite-containing cosmid clones were isolated by screening a pig cosmid library with a (CA)(10) probe and were regionally mapped to Chrs 2, 6, 7, 13, and 14. Ten of the 19 chromosomes in the pig were labeled with these probes. Two-color fluorescence in situ hybridization was used to increase the efficiency of the cosmid localizations.

引用

页码：34 / 37

页数：4

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