HIGH-PERFORMANCE LATEX REAGENT FOR HYDATID SEROLOGY USING AN ECHINOCOCCUS-GRANULOSUS LIPOPROTEIN ANTIGEN FRACTION PURIFIED FROM CYST FLUID IN ONE-STEP

被引：21

作者：

BARBIERI, M ^{[1
]}

STERLA, S ^{[1
]}

BATTISTONI, J ^{[1
]}

NIETO, A ^{[1
]}

机构：

[1] UNIV REPUBL,INMUNOL LAB,SALTO,URUGUAY

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 05期

关键词：

ECHINOCOCCUS-GRANULOSUS; HYDATID DISEASE; HYDATIDOSIS; LIPOPROTEIN ANTIGENS; LATEX AGGLUTINATION; ELISA; LIPOPROTEIN GLYCOSAMINOGLYCANS INTERACTION;

D O I：

10.1016/0020-7519(93)90161-Q

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A lipoprotein fraction from fertile bovine hydatid cyst fluid (FBHCF) was isolated by affinity chromatography on acrylic-heparin. The heparin-binding lipoprotein fraction (HBLF) was proved to be free from host immunoglobulins and albumin. Immunoblotting with confirmed human hydatid sera showed that the major relevant diagnostic bands in FBHCF were also present in HBLF. HBLF or FBHCF were used to sensitize polystyrene latex particles. HBLF-latex showed both higher reactivity with positive hydatid human sera and higher batch-to-batch reproducibility than FBHCF-latex. Titration of sera from 119 surgically confirmed hydatid patients, 48 with other parasitic diseases, 6 with unrelated diseases and 37 healthy subjects was performed using HBLF-latex and conventional ELISA (using FBHCF). Sensitivity and specificity were 83 and 87% for ELISA and 87 and 88% for HBLF-latex. These results indicate that, while being simpler and faster to perform, the HBLF-latex has a similar diagnostic value to that of the conventional ELISA test.

引用

页码：565 / 572

页数：8

共 38 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF SPECIES-SPECIFIC AND CROSS-REACTIVE ANTIGENS OF ECHINOCOCCUS-GRANULOSUS CYST FLUID [J].