RECOVERY OF A BACTERIAL SUBPOPULATION FROM SEWAGE USING IMMUNOFLUORESCENT FLOW-CYTOMETRY AND CELL SORTING

被引：15

作者：

PORTER, J ^{[1
]}

ROBINSON, J ^{[1
]}

PICKUP, R ^{[1
]}

EDWARDS, C ^{[1
]}

机构：

[1] INST FRESHWATER ECOL,AMBLESIDE,CUMBRIA,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 1-2期

关键词：

FLOW CYTOMETRY; CELL SORTING; OCHROBACTRUM ANTHROPI;

D O I：

10.1111/j.1574-6968.1995.tb07883.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin-G-binding particles present at low levels in sewage outflow samples taken from Coniston Water, Fluorescence-activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi.

引用

页码：195 / 199

页数：5

共 10 条

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