A RADIOCHEMICAL METHOD FOR MONOAMINE OXIDASE ASSAY

被引：8

作者：

FELLMAN, JH

ROTH, ES

MOLLICA, RF

机构：

[1] Department of Biochemistry, Division of Neurology, University of Oregon Medical School, Portland

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90126-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple radiochemical method is described for the assay of monoamine oxidase utilizing tritiated tyramine. The synthesis of tyramine-1,2-3H from tyrosine-2,3-3H is reported. Tritium, released by the oxidation of tyramine-1,2-3H by monoamine oxidase, was counted by conventional liquid scintillation techniques. Also reported is the application of this method for the study of a number of parameters which influence the activity of the enzyme, including pH, substrate concentration, and amount of enzyme. © 1969.

引用

页码：339 / &

共 11 条

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