ISOLATION AND CHARACTERIZATION OF A LARVAL LIPOPROTEIN FROM THE HEMOLYMPH OF MANDUCA-SEXTA

被引：97

作者：

PATTNAIK, NM ^{[1
]}

MUNDALL, EC ^{[1
]}

TRAMBUSTI, BG ^{[1
]}

LAW, JH ^{[1
]}

KEZDY, FJ ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT BIOCHEM,CHICAGO,IL 60637

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1979年 / 63卷 / 04期

关键词：

D O I：

10.1016/0305-0491(79)90048-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The larval high density lipoprotein (HDL) from the hemolymph of Manduca sexta, isolated by density gradient centrifugation, contains 61% protein, 37% lipid and 2% carbohydrate. 2. 2. The molecular weight of HDL is 6 × 105, with two apoproteins of 2.85 × 105 and 8.1 × 104 daltons. 3. 3. The large apoprotein is destroyed by trypsin treatment of the particle, while the small one is not. 4. 4. Calculations based upon size and composition show that this particle is very different in structure from mammalian lipoproteins. It is proposed that a portion of the apoprotein occupies the central core region. © 1979.

引用

页码：469 / 476

页数：8

共 29 条

[1]

BARTLETT GR, 1959, J BIOL CHEM, V234, P466

[2]

BLISS CI, 1967, STATISTICS BIOL, V1, P92

[3] STRUCTURE OF HUMAN DENSITY LIPOPROTEIN - A STUDY OF EFFECT OF PHOSPHOLIPASE A AND TRYPSIN ON ITS COMPONENTS AND OF BEHAVIOR OF LIPID AND PROTEIN MOIETIES AT AIR-WATER INTERPHASE [J].