ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR IGG AND IGE ANTIBODIES TO PROTEIN AND POLYSACCHARIDE ANTIGENS OF ASPERGILLUS-FUMIGATUS

被引:175
作者
SEPULVEDA, R [1 ]
LONGBOTTOM, JL [1 ]
PEPYS, J [1 ]
机构
[1] BROMPTON HOSP,INST CARDIOTHORAC,DEPT CLIN IMMUNOL,LONDON SW3 6HP,ENGLAND
来源
CLINICAL ALLERGY | 1979年 / 9卷 / 04期
关键词
D O I
10.1111/j.1365-2222.1979.tb02494.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
ELISA has emerged as a useful alternative to other more costly and complex tests. Polystyrene microhaemagglutination plates have been used as solid phase to absorb Aspergillus fumigatus protein and polysaccharide components for detection of specific antibodies in patients with various forms of pulmonary aspergillosis. IgG and IgE antibodies to the polysaccharide as well as the protein allergens have been found. For the IgE test a double antibody technique has been developed, which is more sensitive than the conventional indirect ELISA. Copyright © 1979, Wiley Blackwell. All rights reserved
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页码:359 / 371
页数:13
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