CHARACTERIZATION OF RECOMBINANT PROTEINS

被引:33
作者
SCOBLE, HA [1 ]
MARTIN, SA [1 ]
机构
[1] GENET INST INC, ANDOVER, MA 01810 USA
关键词
D O I
10.1016/0076-6879(90)93436-O
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This chapter discusses characterization of recombinant proteins. The therapeutic utility of a recombinant protein is profoundly influenced by its macromolecular structure. The structural features that influence biological response may vary widely depending on the cell type used as the host expression system and the conditions under which the protein is expressed. Variation in biological response (in vitro or in vivo) may be attributed to the primary structure of the protein or to posttranslational modification or processing by the host expression system. Indeed, even within a specific cell line, a number of physiological and nonphysiological conditions influence the extent and type of posttranslational processing, and, hence, the therapeutic utility of the expressed protein. In order to better understand the functional implications of structural variation, it is important to assess in detail the structural features of recombinant proteins. These structural features include both host-specific modifications (e.g., glycosylation, acylation, and phosphorylation) and those that may be induced during expression or purification (e.g., oxidation, deamidation, and N- and C-terminal heterogeneity). © 1990, Elsevier Inc. All rights reserved.
引用
收藏
页码:519 / 536
页数:18
相关论文
共 15 条
[1]   FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY [J].
BARBER, M ;
BORDOLI, RS ;
SEDGWICK, RD ;
TYLER, AN .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) :325-327
[2]   CHARACTERIZATION BY TANDEM MASS-SPECTROMETRY OF STRUCTURAL MODIFICATIONS IN PROTEINS [J].
BIEMANN, K ;
SCOBLE, HA .
SCIENCE, 1987, 237 (4818) :992-998
[3]   SEQUENCING OF PEPTIDES BY TANDEM MASS-SPECTROMETRY AND HIGH-ENERGY COLLISION-INDUCED DISSOCIATION [J].
BIEMANN, K .
METHODS IN ENZYMOLOGY, 1990, 193 :455-479
[4]   MASS-SPECTROMETRIC METHODS FOR PROTEIN SEQUENCING [J].
BIEMANN, K .
ANALYTICAL CHEMISTRY, 1986, 58 (13) :1288-&
[5]   IDENTIFICATION OF ATTACHMENT SITES AND STRUCTURAL CLASSES OF ASPARAGINE-LINKED CARBOHYDRATES IN GLYCOPROTEINS [J].
CARR, SA ;
BARR, JR ;
ROBERTS, GD ;
ANUMULA, KR ;
TAYLOR, PB .
METHODS IN ENZYMOLOGY, 1990, 193 :501-518
[6]  
CARR SA, 1988, ANAL PEPTIDES PROTEI, P95
[7]   MATRIX SELECTION FOR LIQUID SECONDARY-ION AND FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY [J].
DEPAUW, E .
METHODS IN ENZYMOLOGY, 1990, 193 :201-214
[8]  
HUNT D F, 1990, P169
[9]  
MARTIN SA, 1988, ANAL PEPTIDES PROTEI, P135
[10]   HYDROPHOBIC EFFECTS IN THE FAST ATOM BOMBARDMENT MASS-SPECTRA OF PROTEINS AND LARGE PEPTIDES [J].
NAYLOR, S ;
MONETI, G ;
GUYAN, S .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 17 (05) :393-397