H-1-NMR STUDIES OF CHROMATIUM-VINOSUM CYTOCHROME-C'

被引：13

作者：

BERTINI, I ^{[1
]}

BRIGANTI, F ^{[1
]}

MONNANNI, R ^{[1
]}

SCOZZAFAVA, A ^{[1
]}

CARLOZZI, P ^{[1
]}

MATERASSI, R ^{[1
]}

机构：

[1] UNIV FLORENCE,CTR STUDY AUTOTROPH MICROORGANISMS,I-50121 FLORENCE,ITALY

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 282卷 / 01期

关键词：

D O I：

10.1016/0003-9861(90)90090-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytochrome c′ from Chromatium vinosum has been studied through 1H NMR in the pH range 4-11 in both the oxidized and the reduced forms. The 1H NMR spectra are similar to those of the other cytochrome c′ systems. Three pKa values of 5.1, 7.0, and 9.2 have been observed for the oxidized species and tentatively assigned to the two carboxylate propionic residues of the heme moiety and to the iron-coordinated histidine 125, respectively. The spectra are consistent with an essentially S = 5 2 state in all the pH ranges investigated. Some evidence is provided for conformational flexibilities. Among the oxidized cytochromes c′ the present one is capable of binding cyanide, giving rise to a low spin state. The reduced species is a typical high spin iron(II) System. © 1990.

引用

页码：84 / 90

页数：7

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