HYPERTONIC SUCROSE TREATMENT ENHANCES 2ND MESSENGER ACCUMULATION IN VASOPRESSIN-SENSITIVE CELLS

被引:19
作者
LUTZ, W
KUMAR, R
机构
[1] MAYO CLIN & MAYO FDN, DEPT MED, NEPHROL RES UNIT, 911A GUGGENHEIM BLDG, ROCHESTER, MN 55905 USA
[2] MAYO CLIN & MAYO FDN, DEPT BIOCHEM & MOLEC BIOL, ROCHESTER, MN 55905 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 02期
关键词
RECEPTOR-MEDIATED ENDOCYTOSIS;
D O I
10.1152/ajprenal.1993.264.2.F228
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
When we treated vasopressin-sensitive vascular smooth muscle cells (A10 cells) and kidney cells (LLC-PK1 cells) with hypertonic sucrose and vasopressin, we observed a decrease in the internalization of radiolabeled vasopressin into these cells and an increase in the amount of second messenger present in these cells following vasopressin stimulation. In A10 cells exposed to hypertonic sucrose, there was an increase in the amount of cellular inositol phosphates accumulated in response to vasopressin compared with cells not exposed to hypertonic sucrose. Similarly, in hypertonic sucrose-treated LLC-PK1 cells, vasopressin increased the amount of adenosine 3',5'-cyclic monophosphate (cAMP) present within the cells compared with LLC-PK1 cells not exposed to hypertonic sucrose. Hypertonic sucrose treatment inhibited receptor-mediated endocytosis in both these cell lines. Hypertonic sucrose alone did not increase the basal amounts of inositol phosphates or cAMP present in A10 or LLC-PK1 cells, respectively. Hypertonic sucrose treatment inhibits receptor endocytosis and increases second messenger concentrations in vasopressin-sensitive cells.
引用
收藏
页码:F228 / F233
页数:6
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