A PUTATIVE TRANSCRIPTION FACTOR-BINDING TO THE UPSTREAM REGION OF THE PUF OPERON IN RHODOBACTER-SPHAEROIDES

被引：11

作者：

SHIMADA, H ^{[1
]}

OHTA, H ^{[1
]}

MASUDA, T ^{[1
]}

SHIOI, Y ^{[1
]}

TAKAMIYA, K ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOL SCI,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

FEBS LETTERS | 1993年 / 328卷 / 1-2期

关键词：

DNA BINDING PROTEIN; GEL SHIFT ASSAY; PUF OPERON; PHOTOSYNTHETIC BACTERIA; RHODOBACTER-SPHAEROIDES;

D O I：

10.1016/0014-5793(93)80961-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gel shift assays of the upstream region of the puf operon in Rhodobacter sphaeroides were performed using cell-free extracts from cells grown under various culture conditions. The results suggested that a protein binding to the upstream region functioned as a repressor-like substance of the expression of the operon by oxygen tension or light. The density of the shifted band of cell-free extracts from cells irradiated with blue light under semi-aerobic conditions was higher than that with red light. Phosphatase treatment of the cell-free extracts strongly increased the DNA-binding affinity of the protein.

引用

页码：41 / 44

页数：4

共 18 条

[1] RHODOBACTER-CAPSULATUS-PUF OPERON ENCODES A REGULATORY PROTEIN (PUFQ) FOR BACTERIOCHLOROPHYLL BIOSYNTHESIS [J].