NETWORK ANALYSIS - ACOUSTIC ENERGY TRANSMISSION DETECTION OF POLYNUCLEOTIDE HYBRIDIZATION AT THE SENSOR LIQUID INTERFACE

被引：31

作者：

SU, HB ^{[1
]}

YANG, MS ^{[1
]}

KALLURY, KMR ^{[1
]}

THOMPSON, M ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT CHEM,80 ST GEORGE ST,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

ANALYST | 1993年 / 118卷 / 03期

关键词：

POLYNUCLEOTIDE HYBRIDIZATION; ACOUSTIC WAVE SENSOR;

D O I：

10.1039/an9931800309

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Palladium has been sputtered onto the surface of gold-plated thickness-shear mode acoustic wave sensors. Adsorption of polynucleotides onto the PdO surface is confirmed by X-ray photoelectron spectroscopy and by gas-phase sensor measurements. Hybridization of complementary strands of polynucleotide at the device-liquid interface results in series resonant frequency signals (network analysis) that are significantly higher than expected from mass-based responses. This observation is interpreted in terms of the perturbation of acoustic energy transmission by changes in interfacial properties on hybridization. Preliminary measurements of changes in motional resistance from network analysis are also presented.

引用

页码：309 / 312

页数：4

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