CONCURRENT LIQUID-CHROMATOGRAPHIC MEASUREMENT OF FLUOXETINE, AMITRIPTYLINE, IMIPRAMINE, AND THEIR ACTIVE METABOLITES NORFLUOXETINE, NORTRIPTYLINE, AND DESIPRAMINE IN PLASMA

被引:23
作者
ELYAZIGI, A
RAINES, DA
机构
[1] Pharmacokinetics Laboratory, Pharmacology and Toxicology Section, Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh
关键词
ANTIDEPRESSANTS; FLUOXETINE; DEPRESSIVE DISORDERS; METABOLITES; PLASMA LEVELS;
D O I
10.1097/00007691-199308000-00008
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
An expedient and specific liquid chromatographic method for a concurrent measurement of fluoxetine (FLU), norfluoxetine (NFLU), amitriptyline (AMI), nortriptyline (NTRIP), imipramine (IMI), and desipramine (DES) is described. Using a mixture of acetonitrile:methanol:0.056 M ammonium acetate: 1 M ammonium hydroxide (100: 10:4.5:2.6, by volume) as mobile phase, the compounds along with doxepin (DOX) (internal standard) were separated on a 10 mu, 8 mm x 10 cm C18 Resolve cartridge in conjunction with radial compression liquid chromatographic module, and were detected in the effluent spectrophotometrically at 220 nm. A hexane:isoamyl alcohol (98:2, by volume) solution was used for extraction of plasma and the drugs were removed from the organic phase with 0.03% phosphoric acid prior to injection. Under these conditions, no interference in the assay was observed, and the retention times of NFLU, DOX, FLU, AMI, IMI, NTRIP, and DES were 7.8, 11.6, 16, 17.8, 20.9, 31, and 35 min, respectively. The assay was highly linear (r > 0.994), and the within- and between-day coefficient of variance was consistently less-than-or-equal-to 9.8%. This assay is currently being used to simultaneously measure these drugs in patients and to investigate their steady-state pharmacokinetics when used in combination.
引用
收藏
页码:305 / 309
页数:5
相关论文
共 13 条
[1]   QUANTIFICATION AND MECHANISM OF THE FLUOXETINE AND TRICYCLIC ANTIDEPRESSANT INTERACTION [J].
BERGSTROM, RF ;
PEYTON, AL ;
LEMBERGER, L .
CLINICAL PHARMACOLOGY & THERAPEUTICS, 1992, 51 (03) :239-248
[2]   CLINICAL-PHARMACOLOGY AND PHARMACOKINETICS OF FLUOXETINE - A REVIEW [J].
BERGSTROM, RF ;
LEMBERGER, L ;
FARID, NA ;
WOLEN, RL .
BRITISH JOURNAL OF PSYCHIATRY, 1988, 153 :47-50
[3]  
DOWNS JM, 1989, J CLIN PSYCHIAT, V50, P226
[4]  
DOWNS JM, 1990, AM J PSYCHIAT, V147, P1251
[5]   TRICYCLIC ANTIDEPRESSANTS ANALYSIS BY LIQUID-CHROMATOGRAPHY [J].
FAZIO, A ;
SPINA, E ;
PISANI, F .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (2-3) :223-240
[6]  
LENSMEYER GL, 1984, CLIN CHEM, V30, P1174
[7]   SIMULTANEOUS QUANTITATION OF 8 TRICYCLIC ANTIDEPRESSANTS IN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
LIN, WN ;
FRADE, PD .
THERAPEUTIC DRUG MONITORING, 1987, 9 (04) :448-455
[8]  
LINET LS, 1989, AM J PSYCHIAT, V146, P803
[9]  
MULLER N, 1991, THER DRUG MONIT, V13, P533
[10]  
ORSULAK PJ, 1988, CLIN CHEM, V34, P1875