FLOW CYTOMETRIC ANALYSIS OF LYMPHOCYTES ISOLATED FROM ENDOSCOPIC BIOPSIES IN ULCERATIVE-COLITIS

被引：4

作者：

BALZANO, A ^{[1
]}

BOVE, A ^{[1
]}

GRANDE, G ^{[1
]}

BEVILACQUA, N ^{[1
]}

机构：

[1] A CARDARELLI HOSP,DEPT GASTROENTEROL,NAPLES,ITALY

来源：

EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY | 1994年 / 6卷 / 03期

关键词：

ULCERATIVE COLITIS; LAMINA PROPRIA; LYMPHOCYTE SUBSETS; ENDOSCOPIC BIOPSIES; FLOW CYTOMETRY; 5-AMINOSALICYLIC ACID; STEROIDS;

D O I：

10.1097/00042737-199403000-00003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objective: In inflammatory bowel disease, surgical specimens are unsuitable for the study of the course of disease and the influence of therapy on lamina propria lymphocytes. The possibility of using per-endoscopic rectal biopsies to overcome this drawback was evaluated in this study. Design: Lymphocytes, identified by flow cytometry, were isolated from rectal biopsies taken during routine endoscopy from patients with ulcerative colitis and from subjects with no signs of acute or chronic bowel inflammation. The resulting data were compared with those from the literature, which were generally derived from total colectomy specimens. The influence of therapy on lamina propria lymphocyte subset distribution was evaluated. Results:The concentration of lamina propria lymphocytes changed significantly in patients with active ulcerative colitis only when compared with controls. The concentrations of CD3+ and CD19+ cells increased despite concurrent 5-aminosalicylic acid or steroid treatment. Activated B (CD3-/HLA-DR+) and T helper (CD4+) cell levels were higher in patients treated with 5-aminosalicylic acid than in all the other groups studied. Activated T lymphocyte (CD3+/HLA-DR+) and T suppressor (CD8+) cell levels were higher in patients with active ulcerative colitis taking steroids than in those with active disease on 5-aminosalicylic acid. Conclusions: This study shows that endoscopic biopsies could be used to study local immunological aspects and to monitor the response to different treatments in ulcerative colitis.

引用

页码：203 / 208

页数：6

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