DNA-POLYMERASE-BETA CAN SUBSTITUTE FOR DNA-POLYMERASE-I IN THE INITIATION OF PLASMID DNA-REPLICATION

被引:18
作者
SWEASY, JB
CHEN, M
LOEB, LA
机构
[1] UNIV WASHINGTON, DEPT BIOCHEM, SEATTLE, WA 98104 USA
[2] UNIV WASHINGTON, DEPT PATHOL, SEATTLE, WA 98195 USA
[3] UNIV WASHINGTON, DEPT BIOCHEM, SEATTLE, WA 98195 USA
关键词
D O I
10.1128/jb.177.10.2923-2925.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We previously demonstrated that mammalian DNA polymerase beta can substitute for DNA polymerase I of Escherichia coli in DNA replication and in base excision repair. We have now obtained genetic evidence suggesting that DNA polymerase beta can substitute for E. coli DNA polymerase I in the initiation of replication of a plasmid containing a pMB1 origin of DNA replication. Specifically, we demonstrate that a plasmid with a pMB1 origin of replication can be maintained in an E. coli polA mutant in the presence of mammalian DNA polymerase beta. Our results suggest that mammalian DNA polymerase beta can substitute for E. coli DNA polymerase I by initiating DNA replication of this plasmid from the 3' OH terminus of the RNA-DNA hybrid at the origin of replication.
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页码:2923 / 2925
页数:3
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