CHARACTERIZATION OF HEMOLYMPH LECTINS IN THE PRAWN PARAPENAEUS-LONGIROSTRIS

被引：25

作者：

FRAGKIADAKIS, G

STRATAKIS, E

机构：

[1] UNIV CRETE,FDN RES & TECHNOL,INST MOLEC BIOL & BIOTECHNOL,GR-71110 IRAKLION,GREECE

[2] UNIV CRETE,DEPT BIOL,GR-71110 IRAKLION,GREECE

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1995年 / 65卷 / 02期

关键词：

PARAPENAEUS LONGIROSTRIS; HEMAGGLUTININ; LECTIN; HEMOLYMPH; CRUSTACEA; N-ACYLAMINOSUGARS; BACTERIA; PSEUDOMONAS AERUGINOSA; ESCHERICHIA COLI; BACILLUS SUBTILLIS; SACCHAROMYCES CEREVISIAE;

D O I：

10.1006/jipa.1995.1017

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Hemagglutinin activity toward mammalian erythrocytes was detected in the hemolymph of the sea prawn Parapenaeus longirostris. Two lectins and one agglutinin were isolated from the hemolymph of this animal after adsorption on formalinized rabbit erythrocytes. The native and the subunit molecular mass estimated with gradient nondenaturing-PAGE and SDS-PAGE was 440 kDa (27-kDa subunit) for one of the lectins, 210 kDa (36-kDa subunit) for the other, and 160 kDa (16- and 18-kDa subunit) for the agglutinin. Both lectins recognized N-acylaminosugars but the 440-kDa lectin was specific for N-acetylneuraminic acid and the 210-kDa lectin for N-acetylgalactosamine. Hemagglutination by P. longirostris lectins and agglutinin required divalent cations, while after EDTA treatment the 440-kDa (27-kDa subunit) lectin dissociated to 60-kDa oligomers. Both lectins and the agglutinin strongly agglutinated formalinized Pseudomonas aeruginosa and Escherichia coli bacteria and may contribute to the defense against specific microorganisms. (C) 1995 Academic Press, Inc.

引用

页码：111 / 117

页数：7

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