BINDING STUDIES BY DIALYSIS EQUILIBRIUM - A DESCRIPTION OF AN ACCURATE AND RAPID TECHNIQUE

被引:17
作者
MCMENAMY, RH
机构
[1] Department of Biochemistry, State University of New York at Buffalo, Buffalo
[2] Department of Surgery, State University of New York at Buffalo, Buffalo
[3] The Edward J. Meyer Memorial Hospital, Buffalo
关键词
D O I
10.1016/0003-2697(68)90016-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple equilibrium dialysis technique is described by which 30 binding studies are conducted simultaneously. Through the use of thin layers of solution with good agitation equilibrium is obtained in 6 hr at 4° or 4 hr at 18°. Methods for washing the membranes, drying the membranes with spacers enclosed, and conducting binding studies are reported. © 1968.
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页码:122 / &
相关论文
共 3 条
[1]  
KRASNER J, 1966, J BIOL CHEM, V241, P1486
[2]  
MCMENAMY RH, 1958, J BIOL CHEM, V233, P1436
[3]  
MCMENAMY RH, IN PRESS