STRUCTURAL PROTEINS OF ADENOVIRUSES .2. PURIFICATION AND CHARACTERIZATION OF ADENOVIRUS TYPE 2 FIBER ANTIGEN

被引：77

作者：

PETTERSSON, UL

PHILIPSON, L

HOGLUND, S

机构：

[1] Division of Cell Biology, The Wallenberg Laboratory, Uppsala University, Uppsala

[2] the Institute of Biochemistry, Uppsala University, Uppsala

来源：

VIROLOGY | 1968年 / 35卷 / 02期

关键词：

D O I：

10.1016/0042-6822(68)90261-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The fiber antigen of adenovirus type 2 has been extensively purified by a five-step procedure comprising Freon extraction, centrifugation on a cushion of RbCl, DEAE-chromatography, isoelectric separation in a pH gradient, and gel filtration on 6% agarose. The final product is homogeneous by analytical ultracentrifugation, polyacrylamide electrophoresis at various pH, immunoelectrophoresis, and electron microscopy. Fiber antigen so prepared has a sedimentation coefficient of 6.1 with a calculated molecular weight of 70,000. Immunodiffusion and hemagglutination revealed two antigenic determinants in the purified fiber, one subgroup specific and the other type specific. The purified fiber did not induce neutralizing antibodies detectable in the plaque assay when it was injected into rabbits. © 1968.

引用

页码：204 / +

页数：1

共 36 条

[1] DETERMINATION OF TYROSINE AND TRYPTOPHAN IN PROTEINS [J].