DEAROMATIZATION OF LIGNIN DERIVATIVES BY FUNGAL PROTOCATECHUATE 3,4-DIOXYGENASE IMMOBILIZED ON POROSITY GLASS

被引：8

作者：

WOJTASWASILEWSKA, M

LUTEREK, J

LEONOWICZ, A

DAWIDOWICZ, A

机构：

[1] MARIE CURIE SKLODOWSKA UNIV,DEPT BIOCHEM,M CURIE SKLODOWSKA SQ 3,PL-20031 LUBLIN,POLAND

[2] MARIE CURIE SKLODOWSKA UNIV,DEPT CHEM PHYS,PL-20031 LUBLIN,POLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1988年 / 32卷 / 04期

关键词：

Biopolymers--Decomposition - Chemical Reactions--Aromatization - Enzymes--Immobilization - Fungi;

D O I：

10.1002/bit.260320413

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protocatechuate 3,4-dioxygenase (see protocatechuate: oxygen 3,4-oxidoreductase, EC 1.13.11.3) was isolated from the mycelium of Pleurotus ostreatus (induced with p-hdroxybenzoic acid) and immobilized on controlled-porosity glass beads, four fractions of Na-lignosulfonates (varying in Mr, after chromatography on Sephadex G-50) were treated with the immobilized enzyme. The products after incubation showed the same Mr as the untreated fractions, but their light absorption at 280 nm, considerably decreased. These studies indicate that dioxygenase causes partial dearomatization of lignin macromolecule.

引用

页码：507 / 511

页数：5

共 27 条

[1] CARBOXYLIC-ACIDS PRODUCED THROUGH OXIDATIVE CLEAVAGE OF AROMATIC RINGS DURING DEGRADATION OF LIGNIN IN SPRUCE WOOD BY PHANEROCHAETE-CHRYSOSPORIUM [J].