APPLICATION OF MICROTITER SOLID-PHASE RADIOIMMUNOASSAY TO THE DETERMINATION OF HEPATITIS-B-E ANTIGEN

被引：7

作者：

MIYAKAWA, Y

TSUDA, F

AKAHANE, Y

MAYUMI, M

机构：

[1] UNIV TOKYO, FAC MED, DEPT INTERNAL MED 3, TOKYO 113, JAPAN

[2] TOKYO METROPOLITAN INST MED SCI, DIV HEPATITIS, TOKYO, JAPAN

[3] JICHI MED SCH, DIV IMMUNOL, TOKYO, JAPAN

来源：

VOX SANGUINIS | 1979年 / 36卷 / 05期

关键词：

D O I：

10.1111/j.1423-0410.1979.tb04439.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Microtiter solid-phase radioimmunoassay (micro-SPRIA) was applied to the detection of hepatitis B e antigen (HBeAg) in [human] serum. Antibody against HBeAg to coat the microtiter plate and for radiolabeling was obtained from the serum of asymptomatic carriers of HB surface [s] Ag by an affinity column of partially purified HBeAg. The micro-SPRIA was sensitive and could be performed without prior concentration of the test serum. HBeAg was detected in 21 (35%) of 60 serum samples positive for HBsAg, all of which were negative by the conventional immunodiffusion method after they were concentrated 3-fold.

引用

页码：307 / 311

页数：5

共 14 条

[1] TYPE B-HEPATITIS - INFECTIVITY OF BLOOD POSITIVE FOR E ANTIGEN AND DNA-POLYMERASE AFTER ACCIDENTAL NEEDLESTICK EXPOSURE [J].