ANTINUCLEAR ANTIBODY DETERMINATION METHODS

被引:4
作者
WILLIAMS, DG
CHARLES, PJ
FIELD, M
CHUA, SM
MAINI, RN
机构
[1] Kennedy Institute of Rheumatology, London
关键词
D O I
10.1007/BF02205552
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Screening rheumatology patients for anti-nuclear and anti-cytoplasmic antibodies is easily done in a qualitative manner using the IF, CIE and ID assays. The immunoblot is of use for anti-La and anti-RNP assays but gives anomalous results for Sm binding by anti-RNP sera and is not easily quantitated. These deficiencies of the immunoblot do not apply to the ELISA. Advances in cloning of autoantigens will enable standardisation of antigen preparations used for these ELISAs. The quantitation of autoantibody appears significant since disease flares occur together with elevations in specific autoantibody. IgM anti- Sm autoantibody was detected with a different disease distribution to IgG anti- Sm but the prognostic implications for this remain to be determined. © 1990 Springer-Verlag.
引用
收藏
页码:51 / 60
页数:10
相关论文
共 44 条
[1]  
Akizuki M., Powers R., Holman H.R., A comparative study of immunological methods for demonstration of antibodies to the soluble nuclear antigens-immunofluorescence, hemagglutination, complement fixation and immunodiffusion, Arthritis Rheum, 20, (1977)
[2]  
D'Arpa P., Machlin P.S., Ratrie H., Rothfield N.F., Cleveland D.W., Earnshaw W.C., cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment, Proc Natl Acad Sci, 85, pp. 2543-2547, (1988)
[3]  
Ben Chetrit E., Chan E.K.L., Sullivan K.F., Tan E.M., A 52kD protein is a novel component of the SS-A/Ro antigenic particle, Journal of Experimental Medicine, 167, pp. 1560-1572, (1988)
[4]  
Ben Chetrit E., Gandy B.J., Tan E.M., Sullivan K.F., Isolation and characterisation of a cDNA clone encoding the 60-kD component of the human SS-A/Ro ribonucleoprotein autoantigen, Journal of Clinical Investigation, 83, pp. 1284-1292, (1989)
[5]  
Chambers J.C., Kenan D., Martin B.J., Keene J.D., Genomic structure and amino acid sequence domains of the human La autoantigen, J Biol Chem, 263, pp. 18043-18051, (1988)
[6]  
Collins R.J., Neil J.C., Druery L.N., Wilson R.J., Detection of antibodies to extractable nuclear antigens using calf thymus and rabbit thymus, J Immunol Methods, 116, pp. 53-57, (1989)
[7]  
Combe B., Rucheton M., Graafland H., Lusiez V., Brunel C., Sany J., Clinical significance of anti-RNP and anti-Sm autoantibodies as determined by immunoblotting and immunoprecipitation in sera from patients with connective tissue diseases, Clin Exp Immunol, 75, pp. 18-24, (1989)
[8]  
Deng J-S., Bair L.W., Medsger T.A., Sontheimer R.A., Correlation between ANA determinations on tissue substrate, Wil-2 cell substrate and precipitin antibody by double diffusionz, Diagnost Clinical Immunol, 5, pp. 151-157, (1987)
[9]  
Deutscher S.L., Harley J.B., Keene J.D., Molecular analysis of the 60- kDa human Ro ribonucleoprotein, Proc Natl Acad Sci., 85, pp. 9489-9483, (1988)
[10]  
Earnshaw W.C., Sullivan K.F., Machlin P.S., Cooke C.A., Kaiser D.A., Pollard T.D., Rothfield N.F., Cleveland D.W., Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen, J Cell Biol, 104, pp. 817-829, (1987)