BIOSYNTHESIS AND HYDROLYSIS OF POLY(GAMMA-GLUTAMIC ACID) FROM BACILLUS-SUBTILIS IFO3335

被引:193
作者
GOTO, A [1 ]
KUNIOKA, M [1 ]
机构
[1] RES INST POLYMERS & TEXT, TSUKUBA, IBARAKI 305, JAPAN
关键词
D O I
10.1271/bbb.56.1031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Poly(gamma-glutamic acid) (PGA) production in Bacillus subtilis IFO3335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA was produced. On the other hand, when glucose was added to the glutamic acid medium, a by-product was produced, which seemed to be a polysaccharide. Moreover, the mode of hydrolysis was investigated with PGA in aqueous solutions at 80, 100, and 120-degrees-C by monitoring the time-dependent changes in the molecular weights. Hydrolytic degradation of PGA was found to proceed through a random chain scission.
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页码:1031 / 1035
页数:5
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