CHARACTERIZATION OF 2 PORCINE PROINSULIN REACTIVE MONOCLONAL-ANTIBODIES BY IMMUNOSTAINING OF BETA-CELLS IN PANCREATIC SECTIONS OF DIFFERENT SPECIES

被引:2
作者
AUGSTEIN, P
BRAUN, B
ZIEGLER, B
WOLTANSKI, KP
ZIEGLER, M
机构
[1] Institute of Diabetes “Gerhardt Katsch”, University of Greifswald, Karlsburg
关键词
D O I
10.1016/S0065-1281(11)80112-1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Murine monoclonal antibodies against porcine proinsulin were generated by somatic cell hybridization. As detected by radioimmunoassay, 2 monoclonal antibodies KSPI14D4 and KSPI13G10 showed a strong binding to I-125-labelled porcine proinsulin but not to insulin. The species specificity of these 2 monoclonals was found to be different as shown by indirect immunofluorescence using sections of Bouin-fixed pancreata of different species. The KSPI14D4 recognized the proinsulin of pig, mouse, man, cattle, rat, dog, and cat but not that of guinea pig, whereas the KSPI13G10 bound to porcine proinsulin only. From these results it is concluded that KSPI14D4 effectively recognizes a wide-spread epitope located in one of the insulin-C-peptide junctions of the proinsulin molecule, whereas KSPI13G10 is directed to a species-specific epitope of the porcine connecting peptide.
引用
收藏
页码:433 / 440
页数:8
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