ANCIENT DNA - METHODOLOGICAL CHALLENGES

被引：219

作者：

HANDT, O

HOSS, M

KRINGS, M

PAABO, S

机构：

[1] Zoological Institute, University of Munich, Munich, D-80021

来源：

EXPERIENTIA | 1994年 / 50卷 / 06期

关键词：

ANCIENT DNA; MOLECULAR ARCHAEOLOGY; PCR; CONTAMINATION; DNA DAMAGE; DNA QUANTITATION; INHIBITION; JUMPING PCR;

D O I：

10.1007/BF01921720

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The study of ancient DNA offers the possibility of following genetic change over time. However, the field is plagued by a problem which is unique in molecular biology - the difficulty of verifying results by reproduction. Some of the reasons for this art technical and derive from the low copy number and damaged state of ancient DNA molecules. Other reasons are the unique nature of many of the objects from which DNA is extracted. We describe methodological approaches with which these problems can be alleviated in order to ensure that results are scientific in the sense that they can be reproduced by others.

引用

页码：524 / 529

页数：6

共 23 条

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