MOLECULAR AND IMMUNOLOGICAL CHARACTERIZATION OF THE P83/100 PROTEIN OF VARIOUS BORRELIA-BURGDORFERI SENSU-LATO STRAINS

被引：28

作者：

ROSSLER, D

EIFFERT, H

JAURISHEIPKE, S

LEHNERT, G

PREACMURSIC, V

TEEPE, J

SCHLOTT, T

SOUTSCHEK, E

WILSKE, B

机构：

[1] UNIV MUNICH,MAX VON PETTENKOFER INST HYG & MED MICROBIOL,D-80336 MUNICH,GERMANY

[2] UNIV GOTTINGEN,ZENTRUM HYG & HUMANGENET,MED MIKROBIOL ABT,D-37075 GOTTINGEN,GERMANY

[3] MIKROGEN GMBH,D-80339 MUNICH,GERMANY

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 1995年 / 184卷 / 01期

关键词：

LYME BORRELIOSIS; BORRELIA BURGDORFERI SENSU LATO; P83/100; ANTIGEN; MOLECULAR ANALYSIS; PHENOTYPIC ANALYSIS;

D O I：

10.1007/BF00216786

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The complete coding regions of the chromosomally encoded p83/100 protein of four Borrelia garinii strains and one Borrelia burgdorferi sensu stricto strain have been amplified by the polymerase chain reaction (PCR), cloned and sequenced. From alignment studies with the deduced amino acid sequences presented here, and five other published p83/100 sequences, the most heterologous region of the p83/100 molecule was identified to be located between amino acid position 390-540. To study the structure of this heterogeneous region, and internal fragment of the p83/100 genes from 11 additional B. burgdorferi sensu late strains was amplified by PCR. The PCR products were analyzed by DNA sequencing and restriction enzyme analysis. These internal p83/100 fragments varied in size and sequence. Cluster analysis of internal p83/100 fragments, as well as restriction enzyme analysis, revealed three major groups in accordance with grouping into the three species causing Lyme disease. Strains within the same species (six B. burgdorferi sensu stricto and six B. afzelii strains) showed similar p83/100 partial structures. Nevertheless, nine B. garinii strains showed more sequence variations and could be further divided into two major subgroups. One group is represented by OspA serotype 4 strains, the other more heterogeneous group is represented by OspA serotypes 3, 5, 6 and 7 strains. Phenotypic analysis with four p83/100-specific monoclonal antibodies revealed four distinct reactivity patterns. Antibody L100 1B4 recognized a common epitope of B. burgdorferi sensu stricto and B. afzelii. Antibodies L100 17D3 and L100 18B4 were reactive with an epitope shared by strains of all three species. The broadest reactivity was shown by L100 18B4 which, in constrast to L100 17D3, additionally recognized the relapsing fever borreliae B. turicatae and B. hermsii. L100 8B8 detected a subgroup of the B. burgdorferi sensu stricto strains. Since comparison of the p83/100 molecule with sequences from protein databases showed similarities with characteristics of eukaryotic cell structures, the p83/100 might mimic these structures and may, therefore, be involved in the immune escape mechanism of the pathogenic agent of Lyme disease.

引用

页码：23 / 32

页数：10

共 41 条

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