MEASUREMENT OF MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION - SELECTIVE-INHIBITION OF ADENYLATE KINASE-ACTIVITY BY P1,P5-DI-(ADENOSINE-5')-PENTAPHOSPHATE

被引：20

作者：

MELNICK, RL ^{[1
]}

RUBENSTEIN, CP ^{[1
]}

MOTZKIN, SM ^{[1
]}

机构：

[1] POLYTECH INST NEW YORK,MICROWAVE RES INST,DEPT ELECT ENGN,BROOKLYN,NY 11201

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90546-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A biochemical assay for the measurement of ATP synthesis coupled to electron transport in the presence of adenylate kinase was developed as an alternative to using the conventional Clark-type oxygen electrode. The assay utilizes P1,P5-di-(adenosine-5′)-pentaphosphate which is shown to be a competitive inhibitor with MgADP for rat liver mitochondrial adenylate kinase (Ki = 7.04 × 10-8 m) and was found to have no effect on oxidative phosphorylation of either intact mitochondria or submitochondrial particles. © 1979.

引用

页码：7 / 11

页数：5

共 18 条

[1] ADENYLATE KINASE-2, A MITOCHONDRIAL ENZYME [J].

BRUNS, GAP ;

REGINA, VM .

BIOCHEMICAL GENETICS, 1977, 15 (5-6) :477-486

[2]

CHANCE B, 1955, J BIOL CHEM, V217, P383

[3]

CRISS WE, 1970, J BIOL CHEM, V245, P6352

[4] SYNTHETIC INHIBITORS OF ADENYLATE KINASES IN ASSAYS FOR ATPASES AND PHOSPHOKINASES [J].

FELDHAUS, P ;

FROHLICH, T ;

GOODY, RS ;

ISAKOV, M ;

SCHIRMER, RH .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 57 (01) :197-204

[5]

GORNALL AG, 1949, J BIOL CHEM, V177, P751

[6]

KHOO JC, 1970, J BIOL CHEM, V245, P4163

[7]

KIELLEY WW, 1958, J BIOL CHEM, V230, P521

[8]

Kuby S.A., 1962, ENZYMES, V6, P515

[9]

LEINHARD GE, 1973, J BIOL CHEM, V248, P1121

[10]

LUSTORFF J, 1976, EXPERIENTIA, V15, P298

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