IMMOBILIZATION OF GLUCOSE-OXIDASE ON NYLON MEMBRANES AND ITS APPLICATION IN A FLOW-THROUGH GLUCOSE REACTOR

被引：12

作者：

DASILVA, MA ^{[1
]}

GIL, MH ^{[1
]}

REDINHA, JS ^{[1
]}

BRETT, AMO ^{[1
]}

PEREIRA, JLC ^{[1
]}

机构：

[1] UNIV COIMBRA,DEPT QUIM,P-3049 COIMBRA,PORTUGAL

来源：

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY | 1991年 / 29卷 / 02期

关键词：

D O I：

10.1002/pola.1991.080290216

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The immobilization of glucose oxidase on hydrolyzed nylon-6, 6 was studied. Various spacers were introduced on the support before the coupling of the enzyme. Best results were obtained when the membrane was covered with denatured bovine serum albumin (BSA) before spacer coupling and immobilization of glucose oxidase (GOD). The influence of various factors (pH, ionic strength, etc.) on the activity of the free and immobilized enzyme was investigated. It was found that the behavior of the fixed glucose oxidase and the free enzyme is very similar. The covalently immobilized enzyme had a lifetime of around 2 months (50% of initial activity).

引用

页码：275 / 279

页数：5

共 9 条

[1]

CZABAN JD, 1985, ANAL CHEM, V57, pA345

[2]

DASILVA MA, 1990, RADIAT PHYS CHEM, V35, P98

[3]

DASILVA MA, 1988, NATO ASI SER, P177

[4] ELECTROCHEMICAL BIOSENSORS [J].

FREW, JE ;

HILL, HAO .

ANALYTICAL CHEMISTRY, 1987, 59 (15) :A933-&

[5] IMMOBILIZED ENZYMES IN ANALYTICAL-CHEMISTRY [J].

GRAY, DN ;

KEYES, MH ;

WATSON, B .

ANALYTICAL CHEMISTRY, 1977, 49 (12) :1067-&

[6]

HUGGETT ASG, 1957, LANCET, V2, P368

[7]

NLI C, 1986, P S ELECTROCHEMICAL, V8614

[8] DESIGN AND DEVELOPMENT OF A FLOW-THROUGH GLUCOSE REACTOR [J].

REDINHA, JS ;

BRETT, AMO ;

PEREIRA, JLC ;

DASILVA, MA ;

GIL, MH .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1989, 22 (03) :379-390

[9]

TURNER APF, 1987, BIOSENSORS

← 1 →